The impact of different carbon and nitrogen sources on antibiotic production by Streptomyces hygroscopicus CH-7

The effects of the composition and rheology of the fermentation medium on the antibiotic production by Streptomyces hygroscopicus CH-7 were studied. To increase the yields of hexaene H-85 and elaiophylin, the basic fermentation medium was reformulated using different carbon (glucose, lactose, ribose, fructose and trehalose) and nitrogen (isatin-Schiff bases) sources. The maximum yields of hexaene H-85 and elaiophylin were obtained with lactose (10 g/l) and soybean (10 g/l) as well as with glucose (10 g/l), soybean (5 g/l) and isatin-3-thiosemicarbazone (5 g/l). The morphology of S. hygroscopicus CH-7 changed with addition of sodium salt of carboxymethylcellulose (CMC) to the basic nutrition medium at the initial concentration between 1.0 and 70.0 g/l, but the microbial growth was dominantly as small dispersed pellets. The production of hexaene H-85 and elaiophylin was stimulated by CMC in the range between 10.0 -30.0 g/l.